In alphabetical order

Adrienne Palicki: Jessica Moore
AJ Buckley: Ed Zeddmore
Allison Hossack: Deanna Campbell
Alona Tal: Joanna Beth Harvelle
Amy Gumenick: Young Mary Campbell Winchester
Chad Lindberg: Ash
Charles Malik Whitfield: FBI Agent Victor Hendrickson
Christopher Heyerdahl: Alastair
Cindy Sampson: Lisa Braeden
Colin Ford: Young Sam Winchester
Fred Lehne: Azazel aka Yellow Eyed Demon
Gabriel Tigerman: Andy Gallagher
Genevieve Padalecki (Cortese): Ruby #2
Jake Abel: Adam Milligan
Jeffrey Dean Morgan: John Winchester
Jim Beaver: Bobby Singer
Julie McNiven: Anna Milton
Katherine Boecher: Lilith
Kurt Fuller: Zachariah
Mark Pellegrino: Lucifer
Mark Sheppard: Crowley
Matthew Cohen: Young John Winchester
Mitch Pileggi: Samuel Campbell
Nicholas Elia: Ben Braeden
Nicki Aycox: Meg Masters
Richard Speight Jr: Trickster aka Gabriel
Ridge Canipe: Young Dean Winchester
Rob Benedict: Chuck Shurley
Robert Wisdom: Uriel
Samantha Ferris: Ellen Harvelle
Samantha Smith: Mary Winchester
Sterling K Brown: Gordon Walker
Traci Dinwiddie: Pamela Barnes
Travis Wester: Harry Spangler